A toxicokinetic study reflecting the absorption, distribution, metabolism and excretion of deoxynivalenol in broiler chickens

To identify the specific biomarkers of exposure DON in chickens, a toxicokinetic study was performed via oral or intravenous application deoxynivalenol (DON). Doses 0.75 and 2.25 mg DON/kg body weight (BW) were administrated intravenously orally to chickens. Next, blood samples collected at several time points plasma obtained. Liquid chromatography–tandem mass-spectrometry (LC–MS/MS) used quantify levels its metabolite DON-3-sulphate (DON-3S). A non-compartmental analysis main parameters after toxin. Regarding administration, level below limit detection (LOD) method (1.5 ng/mL) DON-3S could not be identified. After administration BW, elimination half-life 57.1 47.7 min, respectively, indicating rapid DON. The found broilers exposed intravenously. absence chicken bolus suggests low absorption this mycotoxin. presence indicates that appropriate biomarker